CrispRGold version Version of CrispRGold to compute sgRNAs. CrispRGold is constantly improved. Version 1.1 considers off-target sites with insertions and deletions. sgRNAs with low-efficiency motifs are filtered out. CrispRGold 1.1 (Latest version)

Target organism The target organism is the organism in which the sgRNA will be used. Its genome is essential for the calculation of the specificity of the sgRNAs. Select... Human (Homo sapiens, hg38) - Latest version Human (Homo sapiens, hg19) Mouse (Mus musculus, mm10) - Latest version Mouse (Mus musculus, mm9) Rat (Rattus norvegicus, rn6) Naked mole rat (Heterocephalus glaber, testphase, based on incomplete annotation, hetGla2) Pig (Sus scrofa, susScr11) Chicken (Gallus gallus, galGal5) African clawed frog (Xenopus laevis, xenLae2) Zebrafish (Danio rerio, danRer11) Fly (Drosophila melanogaster, dm6) Worm (Caenorhabditis elegans, ce11)

Design method The design method defines the input you provide. "By gene" requires official gene symbols. Multiple genes can be separated by commas. "By sequence" leads to a search in the provided sequence (name:sequence). By gene name By sequence By sequence (fasta format)

Cloning vector (for ready-to-order oligos) The targeting-vector may be specified to automatically add the cloning over-hangs to the sgRNA cloning sequences. Standard vectors (pX330, lentiCRISPR v2, etc) (BbsI) LKV lentiviral vector (pLKV-U6gRNA(BbsI)-PGKPuroP2ABFP) (BbsI) None (sequences only)

Number of sgRNAs per gene/sequence The number of sgRNAs that will be shown in the output (if identified). This will only affect computation for n > 20. 1 2 3 4 6 10 20 All (slow for large gene sets)

Number of top-risk off-target sites shown The number of off-target sites that will be shown in the output (if identified). This will only affect computation for n > 100. 5 15 20 50 100 All (slow for large gene sets)

Gene names (seperated by comma or linebreak):