CrispRGold

CrispRGold is a sgRNA design tool that searches for optimal sgRNA candidates in given genes or sequences.

CrispRGold goes through iterative loops with decreasing stringency to find sgRNAs. The most important criterion for the design is the specificity, which is calculated based on genome-wide alignments of each candidate sgRNA and subsequent analysis of the mutation distance of each potential off-target site. In addition to the specificity, CrispRGold filters for parameters important for the efficiency.

CrispRGold is free for academic use. For commercial use, please contact us.

For more information, please read the following articles:

sgRNA Sequence Motifs Blocking Efficient CRISPR/Cas9-Mediated Gene Editing.

Graf R, Li X, Chu VT and Rajewsky K.

Cell Reports. 2019 Jan 29.

Read at Cell Reports

Efficient CRISPR-mediated mutagenesis in primary immune cells using CrispRGold and a C57BL/6 Cas9 transgenic mouse line.

Chu VT, Graf R, Wirtz T, Weber T, Favret J, Li X, Petsch K, Tran NT, Sieweke MH, Berek C, Kühn R, Rajewsky K.

Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):12514-12519.

PMID: 27729526

Updates on CrispRGold:

June 10, 2024Safety update. Corrigendum in insertion-position in graphical output of predicted off-targets.

May 28, 2024Update to version 1.2. Server maintenance. Improvement of efficiency. Exclusion of folding energies in sgRNA selection.

January 29, 2019Filtering of low-efficiency motifs implemented in CrispRGold. See publications.

December 20, 2018sgRNAcheck tool has been released for easy check of sgRNA properties and off-target analysis.

April 9, 2018Off-target sites with insertions and deletions are now predicted and included to rank sgRNAs (based on experimental evidence).

January 4, 2018Performance update. In addition, the By sequence search no longer favors sgRNA candidates early in the sequence.

December 21, 2017Most recent mouse (mm10) and human (hg38) genome assemblies added.

November 25, 2017Pig (Sus scrofa), chicken (Gallus gallus), african clawed frog (Xenopus laevis), zebrafish (Danio rerio) and Fly (Drosophila melanogaster) have been added to the target organism.

November 24, 2017The naked mole rat has been added to the target organism. Note that this is based on incomplete sequence and annotation tables. Users are advised to search for specific sgRNAs using the 'by sequence' mode.

November 21, 2017Design update. Minor improvements in data processing.

December 20, 2016Caenorhabditis elegans has been added to the target organism. Bugs removed.

November 12, 2016CrispRGold website goes online.